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  • ContentsContents
  • Article InfoArticle Info
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  • Article title
  • Abstract
  • Key words
  • Introduction
  • Materials and methods
    • Isolation of CD34+ cord blood stem cells
    • In vitro generation of Langerhans cells
    • Flow cytometry
    • Isolation of RNA
    • qRT-PCR
    • Live imaging
    • Bacterial culture
    • Cytokine ELISA
    • Isolation of CD4+ T cells
    • LC – T cell co-culture
    • Statistical analysis
  • Results
    • Heat-killed SA and SE both lead to LC activation
    • Heat-killed SA and SE both promote the secretion of pro-inflammatory cytokines
    • LCs react very differently to living SA than to SE
    • Live SA induces LCs to produce a very different secretome than live SE
    • LCs primed with live SA induce a T-cell response, but LCs primed with SE do not
  • Discussion
  • Acknowledgements
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • References
  • Supplementary material
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